Supported by CCR Office of Science and Technology Resources (OSTR)

Spatial Imaging Technology Resource (SpITR)

Formerly known as the Collaborative Protein Technology Resource (CPTR)
is an open core supported by the Office of Science and Technology Resources (OSTR) dedicated to establishing and implementing cutting-edge molecular profiling technologies to facilitate discovery, translational and clinical research.

Project Management
Publications

Technologies

At SpITR, we offer expertise and provide state-of-the-art technologies to accommodate the needs of CCR/NCI/NIH investigators.

Simple Western

An automated capillary immunoassay system for precise and accurate measurement of proteins and respective post-translational modifications in a format that is applicable to very small sample sizes.

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Luminex xMAP

A multiplex in-solution ELISA analysis system with small sample consumption. The system provides highly quantitative assays for measurement of multiple cytokines, metabolites, and serum/plasma biomarkers in the same sample.

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PhenoCycler(formerly CODEX)

An immunofluorescence imaging platform for highly multiplexed (30-50 plex) IHC-analysis with single-cell resolution and retention of spatial context. It allows comprehensive in-situ protein profiling with multi-parametric readouts from a single tissue section.

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Digital Spatial Profiling (DSP)

Combines standard immunofluorescence techniques with the nCounter® digital optical barcoding technology to perform highly multiplexed, spatially resolved tissue profiling experiments.

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Single Cell Western

Milo is the world’s first Single Cell Western blotting platform that measures protein expression in ~1000 single cells per run using conventional Western blot antibodies.

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CosMx Spatial Imager (SMI)

The most flexible and robust spatial single-cell imaging platform for: Cell Atlas and Characterization: Define cell types, cell states, tissue microenvironment phenotypes, and gene expression networks within spatial context.

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Our Team

Noemi Kedei, MD

Facility Head/Staff Scientist

NIH Building 41, Room D310

Phone: 240-760-6922

kedein@mail.nih.gov

Xiaoling Luo, MS

Biologist

NIH Building 41, Room D310

Phone: 240-760-6932

xiaoling.luo@nih.gov

Maria Hernandez, Ph.D

Biologist

NIH Building 41, Room D310

Phone: 240-760-7774

maria.hernandez2@nih.gov
Address:

41 Medlars Drive

Bldg:

41, Room D310